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Saturday, 28 September 2019

Microscope Slide

In Science, we have also learnt how to set up a microscope slide to be able to see the different organelles under the lens. In this task, we have look through the onion skin.

Basic Slide Preparation Equipment
Stain - A chemical stain is often used to dye the cell (for particular organelles) so they are more visible.
Tweezers - For manipulating the line specimen and coverslip.
Coverslip - A very hin piece of glass used to hold the specimen flat and keep it in place.
Slide - It's made of glass, this is used to hold the specimen.
Specimen - MUst be transparent and thin.

How to set it up: (Making Slide)
1) Place your specimen in the middle of a clean slide. Ensure that the specimen is laying flat and not folded over on itself.

2) Add 2-3 drops of the stain solution. Plant cells are commonly stained with iodine, whereas animal cells are commonly stained with methylene blue. Both of these solutions stain the nucleus of each cell (and your fingers and clothes, so be careful).

3) Holding a coverslip by its edges in your left hand, manoeuvre it so that the bottom edge of the coverslip makes contact with both the slide and the edge of the stain solutions. With your right hand, support the top edge of the coverslip with a pair of tweezers.

4) Gently lower the coverslip with the tweezers, ensuring that no air bubbles are trapped under the coverslip.

5) Place your slide on the microscope stage and examine it under low-power magnification.

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